Vmax™ Express is a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. This rationally engineered, next-generation prokaryotic protein expression system can serve as a replacement for slow growing E. coli systems that are prone to low yields and the expression of proteins as insoluble inclusion bodies. Vmax™ cells are derived from the marine microorganism, Vibrio natriegens. This gram-negative, non-pathogenic bacterium has a doubling time twice that of E. coli and robust transcription and translation systems that support this rapid growth rate. This means that Vmax™ Express can produce greater amounts of biomass and generate large amounts of protein in a shorter time period.
With a protein expression workflow similar to E. coli, Vmax™ Express is compatible with plasmids and antibiotics commonly used with bacterial expression systems such as E. coli BL21(DE3). The Vmax™ Express workflow, fast growth, and ability to express a wide range of proteins make it ideal for routine protein expression. Additionally, Vmax™ Express can help overcome common recombinant protein expression challenges such as low yields or the expression of insoluble, or inactive proteins. Built with a tightly controlled, IPTG-inducible T7 promoter system, Vmax™ Express cells can be cultured using routine growth medium, as well as commercial auto-induction media, or our Vmax™ Enriched Growth Media (recommended for rapid growth and greatest accumulation of biomass).
In contrast to E. coli, Vmax™ Express cells grow rapidly at both 30°C and 37°C and tolerate the induction of protein expression over a wide range of starting ODs and IPTG concentrations. In addition, Vmax™ Express cells can be incubated for up to 24 hours post induction to maximize the amount of soluble protein produced. This flexible protein expression workflow and rapid doubling time allows you to go from transformed cells or a glycerol stock to a large-scale culture ready for analysis or protein purification in as few as three standard workdays compared to four days with E. coli.
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|Protein Expression Strain Performance Attribute||E. Coli Strains||Vmax™ Express|
|Compatible with standard plasmid origins of replication (e.g. pMB1, ColE1, pUC, p15A)||✔||✔|
|Able to use common inexpensive growth media||✔||✔|
|Plasmid selection uses common antibiotic resistance markers (Amp, Tet, Kan, Cm)||✔||✔|
|Tightly controlled IPTG Inducible T7 transcription system||✔||✔|
|Doubling time of ~14 minutes||✖||✔|
|Biomass >14 OD after 24 hours growth||✖||✔|
|Flexibility to induce expression at range of OD600||✖||✔|
|Rapid growth at both 30° and 37°C*||✖||✔|
|Culture ready for protein purification and analysis in 3 days||✖||✔|
|Vmax™ Enriched Growth Media||Liquid Culture||Growth||Growth||Growth||++++|
|Enhanced 2xYT Media||Liquid Culture||Growth||Growth||Growth||+++ Recommended|
|Brain Heart Infusion Broth + v2 salts‡||Liquid Culture||Growth||Growth||Growth||++|
|LB-Miller†||Liquid Culture||Growth||Growth||Supplement with v2 salt‡||++|
|Terrific broth||Liquid Culture||Not tested||Growth||Growth||+|
|MagicMedia™||Liquid Culture||Not tested||Growth||Growth||+|
|LB-Miller†||Agar Plate||Slow Growth||Growth||Growth||+++ Recommended|
|Brain Heart Infusion agar + v2 salts‡||Agar Plate||Growth||Growth||Growth||++|
A variety of commonly used plasmid origins of replication and antibiotic selection markers are compatible with Vmax™ Express. This allows the use of existing expression constructs for recombinant protein expression. After transformation or recovery from a glycerol stock, Vmax™ Express cells can be plated on nutrient agar with appropriate antibiotics. The tables below provide the growth conditions and suggested antibiotic concentrations for both solid and liquid media.
|Plasmid Origin of Replication||Plasmid Backbone||Growth Plate Recommendation|
|pMB1||pBR322 pET vectors||Recover at 30 or 37°C
|Recover at 30 or 37°C
|pUC||pUC19||If using Kan selection, recover at 30°C only.|
|p15A||pACYC184||Recover at 30 or 37°C
|Antibiotic Marker||Concentration (µg/mL) for Solid Media||Concentration (µg/mL) for Liquid Culture|
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