The Gibson Assembly® Ultra Kit
The Gibson Assembly® Ultra Kit provides a robust and efficient method to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This method is designed for complex assembly of 2 to 15 large DNA constructs using only small amounts (nanograms) of DNA.
The Gibson Assembly® Ultra method utilizes a special mix of enzymes to assemble DNA quickly and efficiently in a two-step reaction. Multiple DNA fragments can be joined with 95% cloning efficiency and constructs up to 1000 kb in length can be efficiently assembled by designing multi-stage Gibson Assembly® Ultra reactions.
Summary
- Multiple Inserts — Assembles up to 15 inserts at one time
- Simple Two-Step Process — 80 minute reaction with less than 6 minutes hands-on time
- Optimal for widely varying fragment sizes from 100 bp - 100 kb in length
- Robust and Efficient — Complex assemblies of 95% cloning efficiencies
- Large Constructs — Create constructs up to 1000 kb in length in multi-stage assembly reactions
- Assembles without needing restriction enzyme sites
- Utilize any vector using simple design strategies
- Seamless — Constructs are joined without the presence of intervening and extraneous sequences
- Adaptability — Easily adapted for multiple DNA manipulations,
ss oligonucleotides and assembling repetitive sequences - Cloning Specificity — Enhance cloning specificity of PCR products due to homology matching between amplicon and vector
- Frequently published method — Since 2009, the Gibson Assembly® method has been cited by over 1,200 scientific publications
Resources
How It Works
The Gibson Assembly® Ultra method utilizes a special mix of enzymes to assemble DNA quickly and efficiently in a two-step reaction. Multiple DNA fragments can be joined with 95% cloning efficiency. Constructs up to 1000 kb in length can be efficiently assembled by designing multi-stage Gibson Assembly® Ultra reactions.
To assemble DNA using the Ultra method:
- Design DNA fragments with overlap regions
- Combine the fragments and Ultra Master Mix A==> Master Mix A catalyzes 3’ chew back
- Inactivate Ultra Master Mix A at 75°C
- Anneal DNA fragments at 60°C
- Combine the fragments and Ultra Master Mix B==> Master Mix B catalyzes repair
The resulting construct is double stranded, fully sealed, and can be used immediately for downstream molecular biology applications
Applications
- Multiple Insert Cloning – Clone multiple inserts into a vector without relying on the availability of restriction sites
- Large Fragment Assemblies – Assemble gene clusters and genome-size fragments
- Mutagenesis – Site-directed mutagenesis to make simultaneous changes in a single reaction
- See now the team at JCVI created the first synthetic cell using Gibson Assembly®
- Application Notes
Gibson Assembly® Ultra Provides High Cloning Efficiency
Gibson Assembly® Ultra Kit has been designed to assemble multiple fragments in a simple two-step master mix reaction.
Gibson Assembly® Reagents
| Products | Catalog # | Price |
|---|---|---|
| Gibson Assembly® Ultra Starter Kit | GA1200-S | $85 |
| Gibson Assembly® Ultra Kit, 10 rxn | GA1200-10 | $169 |
| Gibson Assembly® Ultra Kit, 50 rxn | GA1200-50 | $680 |
| Gibson Assembly® Ultra Master Mixes (2X), 10 rxn | GA1200-10MM | $159 |
| Gibson Assembly® Ultra Master Mixes (2X), 50 rxn | GA1200-50MM | $630 |
| Gibson Assembly® Positive Control | GA1000-05C | $63 |
Bulk Packaging
Gibson Assembly® Ultra MasterMixes (2X)
| MasterMix Volume | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MasterMix A | MasterMix B | Product | Catalog # | Total # of reactions | Standard Price | Discount | Discounted Price | Price/Reaction | ||||||
| 5 mL | 10 mL | Gibson Assembly® Ultra MasterMixes (2X) | GA1200-B05 | 1000 | $12,600 | 52.38% | $6,000 | $6.00 | ||||||
| 10 mL | 20 mL | GA1200-B10 | 2000 | $25,200 | 56.35% | $11,000 | $5.50 | |||||||
| 20 mL | 40 mL | GA1200-B20 | 4000 | $50,400 | 60.32% | $20,000 | $5.00 | |||||||
| 30 mL | 60 mL | GA1200-B30 | 6000 | $75,600 | 64.23% | $27,000 | $4.50 | |||||||
| 40 mL | 80 mL | GA1200-B40 | 8000 | $100,800 | 68.25% | $32,000 | $4.00 | |||||||
| 50 mL | 100 mL | GA1200-B50 | 10000 | $126,000 | 72.22% | $35,000 | $3.50 | |||||||
Above mentioned pricing is valid only in the U.S. For international pricing please contact customerservice@sgidna.com.
Prices do not include shipping and handling. Submit by 2pm (PST) to receive order by the next day. For bulk packaged size, lead time is ~3 weeks.
Gibson Assembly method is also available under commercial license, please inquire at info@sgidna.com
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