The Gibson Assembly® Method Overview

One of the essential technologies used by SGI-DNA is the Gibson Assembly® method, a revolutionary method developed by Daniel Gibson and colleagues at the J. Craig Venter Institute (JCVI).

Since its introduction to the life science community in 2009, the Gibson Assembly® method has become a mainstay in many synthetic biology laboratories and has attracted interest from both the academic and commercial life sciences community due to its ease-of-use, robustness and flexibility.

For non-commercial use, SGI-DNA Gibson Assembly® reagents do not require a license and there are never any royalties due. Contact us at with any questions and for licensing the Gibson Assembly® method for commercial use. SGI-DNA is the only provider of Gibson Assembly® commercial licenses, and charges a yearly fee with no royalties. We strive to make Gibson Assembly® easy and convenient to use for all scientific researchers.

Find out why scientists prefer the Gibson Assembly® method – Watch our video!

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Choose Your Gibson Assembly® Kit

Feature Gibson Assembly® HiFi 1-Step Kit Gibson Assembly® Ultra Kit Gibson Assembly® Site Directed Mutagenesis Kit
Hands on Time <5 minutes <6 minutes <40 minutes
Reaction Time 60 minutes 80 minutes 4-6 hours
No. of steps 1 2 3
Size of each fragment 500bp to 32kb 100bp to 100kb 500bp to 5.5kb
Efficiency >90% >95% >95% for single site
>55% for five sites
No. of fragments you can assemble Up to 5 Up to 15 Up to 5
Total construct size 100kb (in multi stage reactions) 1000kb (in multi stage reactions) 50kb (in multi stage reactions)
Key Benefits
  • Quick and easy.
  • Recommended for less than/equal to 5 fragment assembly.
  • Master mix includes Phusion® DNA Polymerase that cuts down on mutations at cloning junctions.
  • More robust and efficient.
  • Ideal for complex assemblies.
  • Recommended for 6-15 fragments.
  • Ideal for very small fragments (100bp) or very large fragments (100kb).
  • Generate up to 5 mutations (insertions, deletions, or substitutions) in a single reaction
  • Insertions up to 40bp
  • Deletions of any size
  • Single kit for mutagenesis and cloning

For more information, please contact:

Customer Service
Phone: 1-855-474-4362