Gibson Assembly® Reagents

The Gibson Assembly® Method Overview

The Gibson Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.

Why scientists prefer the Gibson Assembly® method

SGI-DNA offers several Gibson Assembly cloning kits for DNA assembly.

Each kit contains specific master mix reagents and proof-reading polymerase.

Gibson Assembly® Reagent Kits

SGI-DNA offers several Gibson Assembly cloning kits for DNA assembly. Each kit contains specific master mix reagents and proof-reading polymerase.

Gibson Assembly® HiFi 1-Step Kit

Hands-on time
<5 min
Total reaction time
60 min
Number of steps
1
Fragment size range
500 bp to 32 kb
Cloning efficiency
>90%
Fragments per reaction
up to 5
Maximum construct 
size (multiple-stage reactions)
100 kb

Gibson Assembly® Ultra Kit

Hands-on time
<6 min
Total reaction time
80 min
Number of steps
2
Fragment size range
100 bp to 100 kb
Cloning efficiency
>95%
Fragments per reaction
up to 15
Maximum construct 
size (multiple-stage reactions)
1000 kb

Gibson Assembly® Site-Directed Mutagenesis Kit

Hands-on time
<40 min
Total reaction time
~4-6 hr
Number of steps
3
Fragment size range
500 bp to 5.5 kb
Cloning efficiency
95% single site
Fragments per reaction
up to 5
Maximum construct size (multiple-stage reactions)
25 kb

Gibson Assembly® HiFi 1-Step Kit

Gibson Assembly® Ultra Kit

Gibson Assembly® Site-Directed Mutagenesis Kit

Hands-on time
<5 min
<6 min
<40 min
Total reaction time
60 min
80 min
~4-6 hr
Number of steps
1
2
3
Fragment size range
500 bp to 32 kb
100 bp to 100 kb
500 bp to 5.5 kb
Cloning efficiency
>90%
>95%
95% single site
Fragments per reaction
up to 5
up to 15
up to 5
Maximum construct size (multiple-stage reactions)
100 kb
1000 kb
25 kb

For non-commercial use, SGI-DNA Gibson Assembly® reagents do not require a license. Contact us at info@sgidna.com with any questions and for licensing the Gibson Assembly® method for commercial use. SGI-DNA is the only provider of Gibson Assembly® commercial licenses, and charges a yearly fee with no royalties. We strive to make Gibson Assembly® easy and convenient to use for all researchers.

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