Gibson Assembly® Reagents
The Gibson Assembly® Method Overview
The Gibson Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.
Why scientists prefer the Gibson Assembly® method
SGI-DNA offers several Gibson Assembly cloning kits for DNA assembly.
Each kit contains specific master mix reagents and proof-reading polymerase.
Gibson Assembly® Reagent Kits
SGI-DNA offers several Gibson Assembly cloning kits for DNA assembly. Each kit contains specific master mix reagents and proof-reading polymerase.
Gibson Assembly® HiFi 1-Step Kit

Gibson Assembly® Ultra Kit

Gibson Assembly® Site-Directed Mutagenesis Kit

Gibson Assembly® HiFi 1-Step Kit

Gibson Assembly® Ultra Kit

Gibson Assembly® Site-Directed Mutagenesis Kit

SGI-DNA Gibson Assembly® reagents do not require a license for research use. For licensing information for commercial uses please contact us at info@sgidna.com. SGI-DNA is the only provider of Gibson Assembly® commercial licenses.