Gibson Assembly® Reagents
The Gibson Assembly® Method Overview
The Gibson Assembly method is an efficient and robust cloning procedure that allows the seamless joining of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Through a series of enzymatic steps, overlapping regions are created, annealed, and ligated over the course of an hour, resulting in a final covalently-joined, double-stranded DNA construct.
Why scientists prefer the Gibson Assembly® method
SGI-DNA offers several Gibson Assembly cloning kits for DNA assembly.
Each kit contains specific master mix reagents and proof-reading polymerase.
Gibson Assembly® Reagent Kits
SGI-DNA offers several Gibson Assembly cloning kits for DNA assembly. Each kit contains specific master mix reagents and proof-reading polymerase.
Gibson Assembly® HiFi 1-Step Kit
Gibson Assembly® Ultra Kit
Gibson Assembly® Site-Directed Mutagenesis Kit
Gibson Assembly® HiFi 1-Step Kit
Gibson Assembly® Ultra Kit
Gibson Assembly® Site-Directed Mutagenesis Kit
For non-commercial use, SGI-DNA Gibson Assembly® reagents do not require a license. Contact us at info@sgidna.com with any questions and for licensing the Gibson Assembly® method for commercial use. SGI-DNA is the only provider of Gibson Assembly® commercial licenses, and charges a yearly fee with no royalties. We strive to make Gibson Assembly® easy and convenient to use for all researchers.