Eliminate time consuming restriction cloning, the need to subclone after TA cloning, and avoid recombination-based cloning that adds ‘att sites’. Get the clone you want in the vector you want. Insert multiple fragments in a single round of cloning and never subclone again! The Gibson Assembly® HiFi 1-Step Kits provide a simple and effective alternative to seamlessly construct synthetic genes, genetic pathways, as well as entire genomes. This simple one tube, one step, isothermal approach allows researchers to create large recombinant DNA constructs, with multiple inserts, in 1 hour.
Gibson Assembly® HiFi 1-Step technology utilizes a special mix of enzymes optimized to assemble the DNA quickly and efficiently. Unlike other approaches, SGI-DNA’s Gibson Assembly process utilizes Phusion® High-Fidelity DNA polymerase for 3’ end extension. This results in higher numbers of seamless error free clones ready for multiple downstream applications.
The Gibson Assembly® HiFi 1-Step Kit is available as either a 2X master mix or 4X high concentration (HC) master mix. The 4X Gibson Assembly HiFi HC 1-Step kit requires only 2.5 µL of mix per 10 µL reaction making it ideal for assemblies using less concentrated DNA samples. Using either the original HiFi 1-Step 2X format or the HiFi HC 1-Step 4X format, multiple DNA fragments can be joined with greater than 90% cloning efficiency. Even large constructs up to 100kb in length can be efficiently assembled in multi stage reactions.
Gibson Assembly® method employs an innovative blend of enzymes in a single tube reaction in a 1 hour isothermal reaction. The process begins by designing dsDNA fragments with 20 – 40bp overlapping ends. The DNA fragments are mixed with the Gibson Assembly® mix for assembly.
The resulting construct is a double stranded, fully sealed, and error free molecule that can be used immediately for numerous molecular biology applications such as direct transformation, PCR, RCA, and other molecular biology applications.
Above mentioned pricing is valid only in the U.S. For international pricing please contact firstname.lastname@example.org.
Prices do not include shipping and handling. Submit by 2pm (PST) to receive order by the next day. For bulk packaged size, lead time is ~3 weeks.
Gibson Assembly method is also available under commercial license, please inquire at email@example.com
If you have a promotional code that you want to be applied to your order, please e-mail us the order form at firstname.lastname@example.org